Photolithography is a simple microneedle preparation process that allows precise control of the microneedle size, shape and preparation material. The steps for the preparation of soluble microneedles by soft photolithography: first, the polymer film is paired with a microneedle mold, which is filled by a heated roller, then the filled mold is placed on a flexible substrate, and again the mold is separated by a heated roller, and the microneedles are retained on the backing material to make a microneedle patch. In addition, heated roller curing can be used instead of light curing. Soft lithography preparation of microneedles is similar to the stereoscopic plate stretching method, with the advantages of high efficiency and low cost and good scalability.
For this evaluation, all patches left behind were left in the skin for 10 seconds, then removed and a green tissue marking dye was immediately applied to the skin and subsequently wiped off so that the location of skin penetration could be identified macroscopically. Drug substitutes can be visually perceived within the microneedle insertion site and cannot be wiped off from the surface.
Further studies were conducted to assess the complete dissolution of the microneedle patch and the release of the drug substitute. For these studies, all patches were left in the skin for 10 minutes, followed by a few drops of water on the back of the microneedle patch.
The tested skin samples were then embedded in optimal cutting temperature media and frozen sections . Half of the sections were set aside for imaging using fluorescence microscopy. These sections were fixed briefly in the cancer diagnostic for 10 seconds prior to capping. The remaining sections were stained for bright-field microscopic imaging, using Cancer Diagnostics' procedures prior to coverslip.
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